Codification : M-AN
Version : B
Date :28/10/2005
. 1/2

-41

MODE OPERATOIRE D'ANALYSE POUR LA CONFIRMATION DE L'ORIGINE DES
METABOLITES DE LA TESTOSTERONE PAR CPG/Sv£l

r

i

D
[

r
Lj

1

u

n
i

u

p

n
U

r
L

D

u

L

I •

LJ

J

COLONNK

Type:
Longueur:
Diametre interne:
ipaisseur du film:

INJECTION

Mode:

Temperature injecteur:
Volume injected
Solvants de rincage ALS:

CONDITIONS CC

Temperature initiale:
Gradient de temperature:

Temperature finale:
Temps d'analyse:
Pression constantc:

INTERFACE

Piege a eau:
Ligne de transfert:
Four a combustion:

CONDITIONS SM
Mode d'acquisition:
Programmation de l'acquisition:

DB17-MS JW Scien 122.4732
30m
0.25mm

0.25um ASSURANCE QUALITY

LNDD

Splitless (insert splitless)

280°C

l|il-4|d

Solvant A: Acetonitrile

Solvant B: Hexane APPLICABLE

le

2 * OCT. 2005

70°C pendant 1 min
70->27rCa30°C/min
271?C->281?C a 0.6°C/min
281°C pendant 3 min
281-^300°Ca5 ,, C/min
300°C pendant5min
45 min
Ajuster le SI a environ 870s

-100°G

350°C

850°C

toNFlOQi

riSL

ions 44, 45 et 46

Temps d'acquiqition total:2580s

a 100s RG open

a 130s RG close

a 160s RG open

a 190s RG close

a 220s RG open

a 250s RG close

a 750s HS close

a 2000s HS open

a 2400s RG open

a 2430s RG close

a 2460s RG open

a 2490s RG close

a 2510s RG open

a 2540s RG close

USADA0153

AU%